Development and Validation of Liquid Chromatography Tandem Mass Spectrometry Method and Its Application in Pharmacokinetic Study of Acetamiprid in Sprague-Dawley Rat

Present study was aimed to develop and validate a liquid chromatography spectrometry method and pharmacokinetic analysis of acetamiprid in Sprague Dawley rat. For the development of pharmacokinetic method, ChemT was used as internal standard. Extraction and recovery of plasma analyte were done using 0.1% formic acid and acetonitrile solution. An eleven point standard curve was prepared by spiking 2μl of working stock of concentration 2ng/ml. Three precision accuracy samples were processed on three separate days. The apparent maximum plasma concentration and the time at which it was achieved were determined. The area under the plasma concentration-time curve was also measured. The elimination rate constant was 0.14±0.06 per hour and the terminal half-life after oral administration were found to be 6.58±1.85h -1 . LCMS/MS method proved to be sensitive and simple tools for PK/TK parameters of any new chemical entities. S. Mondal 1 , S.K. Mukhopadhayay 1 and Rammohan Bera 2 1 Department of Veterinay Pathology, West Bengal University of Animal and fishery Sciences, Kolkata-37 2 Jadavpur University, Kolkata-75, India. Submission: 1 August 2016 Accepted: 7 August 2016 Published: 25 August 2016 www.ijppr.humanjournals.com Citation: S. Mondal et al. Ijppr.Human, 2016; Vol. 7 (1): 263-275. 2 64 264